Characterisation of Pasteurella multocida isolated from fowl cholera outbreaks on turkey farms

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Persistence of Pasteurella multocida in wetlands following avian cholera outbreaks.

Avian cholera, caused by Pasteurella multocida, affects waterbirds across North America and occurs worldwide among various avian species. Once an epizootic begins, contamination of the wetland environment likely facilitates the transmission of P. multocida to susceptible birds. To evaluate the ability of P. multocida serotype-1, the most common serotype associated with avian cholera in waterfow...

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Experimintal Study of Peracute Fowl Cholera due to Pasteurella multocida Vaccinal Strain (serotype A1) in Chickens

In order to show the type and severity of gross and histopathologic lesions induce by vaccinal strain (serotype A1) of Pateurella mulocida, ten four-week-old SPF chickens were inoculated intramuscularly with 75 cfu of ( 0.5 ml of 10-7 dilution) bacterium. All birds died in less than 16 hours. No prominent gross lesions were observed in different organs. In microscopic examination, the most comm...

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Simplified identification of somatic varieties of Pasteurella multocida causing fowl cholera.

Representative type strains as well as field strains of Pasteurella multocida from fowl cholera were examined by the indirect hemagglutination procedure and by a rapid plate agglutination test. The former method identified the specific capsular or K substance, and the latter identified the somatic antigens. Inagglutinable organisms were made agglutinable for the rapid procedure by treatment wit...

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Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A.

We have constructed a defined acapsular mutant in Pasteurella multocida X-73 (serogroup A:1) by disrupting the hexA gene through the insertion of a tetracycline resistance cassette. The genotype of the hexA::tet(M) strain was confirmed by PCR and Southern hybridization, and the acapsular phenotype of this strain was confirmed by electron microscopy. The hexA::tet(M) strain was attenuated in bot...

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Protection against fowl cholera conferred by vaccination with recombinant Pasteurella multocida filamentous hemagglutinin peptides.

Three gene fragments, derived from Pasteurella multocida strain P-1059 (serotype A:3), encoding approximately the 5' one-third of fhaB2 were overexpressed individually in Escherichia coli. The recombinant peptides were purified, pooled, and administered to turkey poults to evaluate immunity. The results showed that turkeys immunized twice with the recombinant peptides were significantly protect...

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ژورنال

عنوان ژورنال: Australian Veterinary Journal

سال: 1995

ISSN: 0005-0423,1751-0813

DOI: 10.1111/j.1751-0813.1995.tb15033.x